Supplement Info on Lab Research on antiviral mRNA, hydrolysis, mRNA, Viperin (ddhCTP) enzyme, etc. Equipment, Suppliers, Companies, and Research Universities or Institutions by Quinton Mitchell

Notes on Labs, Universities associated with Projects, Equipment Suppliers, and Organizations:

  1. Moderna Therapuetics
  2. Sino Biological
  3. Promega
  4. BD Bioscience
  5. ThermoFisher (includes Life Technologies and Fisher Scientific)
  6. JEOL
  7. Qiagen
  8. Agilent
  9. VectorLabs
  10. Mettler Toledo
  11. Hanna Instruments
  12. Intertek
  13. Sigma Aldrich
  14. (Not included in the research I studied) Hemler Scientific (Deep Temperature Freezers)
  15. (Not included in the research I studied) Eppendorf (Electroporation Machines)
  16. The Lab Depot
  1. Penn State University and University of North Carolina on Viperin Antiviral Enzyme Research Twitter: @cameronlabunc
  2. La Trobe University Helbig Lab on Viral Infection
  3. Albert Einstein College of Medicine on Antiviral Viperin Enzymes Research
  4. Stanford University on Exploiting Zika addiction to Hsp70 protein
  5. Stanford University of the OST Zika Research
  6. University of Arizona on Nanotechnology
  7. National Institute of Animal Science, Rural Development Administration; Ajou University School of Medicine, and Sungkyunkwan University on 3D8 research
  8. Rockefeller University
  9. National Institute of Health on Remdesivir
  10. MIGAL (Gallile Research Institute) on IBV research and protein expression vectors
  11. Reagent and Vaccine Services Section (RVSS) of the USDA National Veterinary Services Laboratories’ (NVSL) FADDL located at the Plum Island Animal Disease Center (PIADC) relating to Alkaline Hydrolysis and Heat Treatment to kill viruses

Equipment used for the OST NSI-1 Zika Study by the Stanford Research and Yale Team

  1. Renilla Luciferase Assay system (Promega) for Luciferase expression measurement
  2. 96-well plates
  3. CellTiter-Glo (Promega) for Cell Growth in Culture Measurement
  4. Trypan blue solution for Cell Growth Measurement
  5. Bio-Rad Gene Pulser Xcell electroporator use for electroporation techniques in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs, or DNA to be introduced into the cell (also called electrotransfer)
  6. Perm/Wash buffer (BD Biosciences) Cell permeabilization Perm/Wash buffer (BD Biosciences)
  7. IgG Alexa 488 (Life Technologies) for Incubation
  8. FlowJo 9  for data analysis for Flow Cytometry
  9. GraphPad Prism 7 for Statistical Analysis

Equipment used by the Foreign Animal Disease Diagnostic Laboratory team (Lemire, Rodriguez, and McIntosh) when studying Heat Treatment and Alkaline Hydrolysis in killing viruses:

  1. RNeasy® Mini Kit (Qiagen, Valencia, CA, USA) for RNA Extractions
  2. DNeasy® Blood and Tissue Kit (Qiagen, Valencia, CA, USA) for DNA Extractions
  3. Qiagen QIAquick Spin Miniprep PCR Purification Kit for Plasmid Purification
  4. pBlueScript II plasmid (Agilent Technologies) Plasmid Propagation
  5. pGEM-T Easy vector plasmid (Promega Corp.) for PCR and Plasmid Propagation
  6. QIAquick® PCR Purification kit (Qiagen) for Purification Reverse-transcriptase polymerase chain reaction PCR
  7. VECTASTAIN® ABC-AP KIT. Viral Isolation Straining
  8. PRIMARIA™ coated culture flasks
  9. Spearman-Kärber method was used to calculate titrations
  10. NanoDrop 2000 spectrophotometer (Fisher Scientific, Pittsburg, PA, USA) for DNA quantification
  11. Sequencher® software (Gene Codes) for DNA Contig Sequencing
  12. Kingfisher 96 magnetic particle processor (Thermo Fisher Scientific) Sequencing product purification, contaminant and impurity QC testing
  13. 3730XL DNA sequencer (Applied Biosystems) for Nucleotide Sequencing
  14. Invitrogen-Thermo Fisher Scientific
  15. GeneAmp® EZ rTth RNA PCR kit (Life Technologies, Grand Island, NY, USA)

RNA Sample Amplification:

  1. SmartCycler II (Cepheid, Sunnyvale, CA) with automatic background subtraction on
  2. TaqMan® Fast Virus 1-Step Master Mix (Life Technologies, Grand Island, NY, USA) Applied Biosystems® 7500 Real-Time PCR System (Life Technologies, Grand Island, NY, USA).
  3. Path-ID™ Multiplex One-Step Kit (Life Technologies, Grand Island, NY, USA)

DNA Sample Amplification:

  1. SmartCycler II PCR platform (Cepheid, Sunnyvale, CA, USA)
  2. TaqMan® EZ RT-PCR kit (Life Technologies, Grand Island, NY, USA).

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